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Singuway Nucleic Acid Detection Kit
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Monkeypox Virus (MPV)

Features


  • Rapid - Complete the whole process from extraction to amplification with a minimum of 42 minutes
  • Flexible - 3 specifications fulfill different demands
  • Accurate - The assay sensitivity of this reagent is 500 copies/mL.



Intended Use

Detection kit for Monkeypox virus is designed to detect DNA from Monkeypox virus in specimens such as serum or plasma, vesicular or pustular lesion fluid collected from individuals.

Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae, and causes a human disease that resembles smallpox.


Test Principle

This kit is based on real-time PCR fluorescence technology, and the specific primers and corresponding fluorescent probes target to the conserved region of MPV were designed for highly specific detection. By detecting the fluorescent signals changes, the MPV nucleic acid from specimen can be qualitatively detected.


Specifications

24 Tests/Kit, 48 Tests/Kit, 96 Tests/Kit


Specimen requirement

Serum or plasma, vesicular or pustular lesion fluid.


Components

Name

24 tests/ kit

48 tests/ kit

96tests/ kit

Constituents

PCR Buffer

450μL ×

1 Tube

900μL ×

1 Tube

900μL ×

2 Tubes

Primers, probes, dNTPs

PolyaseMix

36μL×1 Tube

72μL×1 Tube

144μL×1Tubes

DNA polymerase, UDG

Negative Control

400μL×1 Tube

400μL×1 Tube

800μL×1Tubes

Normal Saline

Positive Control

400μL×1 Tube

400μL×1 Tube

800μL×1Tubes

Synthetic nucleic acid with target gene fragment

Applicable Instruments:

Areal-timePCR instrument containingFAM and HEX/VICfluorescence channels, such as Singuway Singu9600 Pro,Bio-RadCFX96, ABI 7500, Thermo Fisher StepOnePlus.



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