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Singuway Nucleic Acid Detection Kit
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Dengue/Zika/Chikungunya Virus Multiplex

Intended Use

Mosquito-borne diseases, generally referred to as diseases that are transmitted by mosquitoes as transmission vectors, spread widely in tropical/subtropical countries; Among them, dengue virus, Zika virus and chikungunya virus have a wide transmission ability, causing various hemorrhagic fevers; Therefore, diagnosis is mandatory.

TheDetection Kit for dengue virus/Zika virus/ chikungunya virus (PCR-Fluorescence Probing) is an in vitro diagnostic test, based on real-time PCR technology, for the detection ofdengue virus/Zika virus/ chikungunya virus nucleic acids inserum samples. The test results are for clinical reference only and cannot be used as a basis for confirming or excluding cases alone. 


Test Principle

This kit is based on real-time PCR fluorescence technology, and the specific primers and corresponding fluorescent probes target to the conserved region ofdengue virus/Zika virus/ chikungunya virus were designed for highly specific detection. By detecting the fluorescent signals changes, thedengue virus/Zika virus/ chikungunya virus nucleic acid from specimen can be qualitatively detected.


Technical Specifications

REF No.

MBD001

Regulatory Status

CE IVD

Packing Specifications

48 tests/kit, 96 tests/kit

Technology

Real-time PCR

Type Of Analysis

Qualitative

Sample Type

Serum

Reaction Volume

30μL

Detecting Target


DENV/CIKV/ZIKV


Analytical Sensitivity

1000 copies/mL

Precision

CV≤5%

Storage

-20±5℃ in dark for 12 months

2~8℃ after opening the cover for2 weeks

Validated Extraction Method

General nucleic acid extraction kit (Singuway Ref No.: MTQ064/MTQ096) is recommended.

Instruments


A real-time PCR instrument containing FAM, HEX/VIC, ROX and Cy5 fluorescence channels, such as Singuway Singu9600, Bio-Rad CFX96, ABI 7500.


Required Detection Channels

FAM, HEX, CY5, ROX

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