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Nucleic Acid Extraction Reagent (Magnetic Bead Method)

Our wide range of magnetic bead kits and reagents help ensure the best balance of high yield and reproducibility with low nonspecific binding, and they can be used for extraction of high quality viral DNA / RNA on instruments for low to high throughput applications.

Extraction Technologies

Magnetic beads bind to nucleic acids (DNA/RNA) or proteins through a specific functional group on the surface. The beads are also easily transferable and removable using the principle of magnetic bead adsorption, thus automatically completing the extraction of nucleic acid. Recently, due to their merits, magnetic beads are universally used in biology application research fields from extraction of biological molecules such as DNA, RNA, proteins and cells to medical research. Singuway’s magnetic beads are uniform, spherical particles with enlarge surface area and superior binding capacity, which enables high-purity, high-yield extraction of DNA, RNA, etc.

Overview of Magnetic-BeadBasedExtraction Product

Regular Nucleic Acid Extraction Reagent (Magnetic Bead Method)

Ref No. &Specifications

l MTQ064 (64 Tests/Kit)

l MTQ096 (96 Tests/Kit)

Features

l Fast extraction: 13-15 minutes to complete the whole process of DNA/RNA extraction.

l Biosafety: No lysis at high temperatures, reducing the formation of aerosols thus reducing the risk of infection to professionals. Does not contain toxic organic reagents such as phenol and trichloromethane.

l Reduced cross-contamination: The reduction of aerosols significantly reduces cross-contamination and false positive results.

l Pre-assembled version: Adaptable to most DNA/RNA extractors on the market.

l High extraction efficiency: Rapid extraction of high purity disease virus DNA/RNA from samples with high sensitivity and high extraction efficiency.

Main Components

Storage Conditions and Validity

The kit is transported and stored at 15-30℃ and valid for 12 months.

Extraction Time

13-15 mins

Applicable Instruments

It is compatible with various nucleic acid extraction instruments such as Singuway Singu32/Singu48/Singu96, Thermo Fisher KingFisher, Tecan, Hamilton, Beckman, Biomek, Genrui Biotech, Bioperfectus, Daan Gene, Zybio, Bioer, etc.

Applicable Test Items

Available for all Singuway test items.

Specimen Requirements

Pharynx swab, nasal swab, nasopharyngeal secretion, serum, plasma, cervical exfoliated cells.

Applications

RT-PCR, cDNA synthesis, Northern/ dot/ slot blot analysis, Microarrays, RNAseq

Product Performance Indicators

Extraction sensitivity:The amplification sensitivity reached ≥100 copies/mL.

Nucleic acid purity: A260/A280 ratio: 1.5~2.2.

Extraction recovery: nucleic acid extraction recovery rate reached 90%~110%.

Fast Nucleic Acid Extraction Reagent (Magnetic Bead Method)

Ref No. &Specifications

l MTQ064F (64 Tests/Kit)

l MTQ096F (96 Tests/Kit)

Features

l High recovery rate of nucleic acid in swab samples

l Minimum of 9 minutes to complete the whole process of DNA/RNA extraction.

l No lysis at high temperatures, reducing the formation of aerosols thus reducing the risk of infection to professionals. Does not contain toxic organic reagents such as phenol and trichloromethane.

l The reduction of aerosols significantly reduces cross-contamination and false positive results.

l Rapid extraction of high purity disease virus DNA/RNA from samples with high sensitivity and high extraction efficiency.

Main Components

Storage Conditions and Validity

The kit is transported and stored at 15-30℃ and valid for 12 months.

Extraction Time

9-19 mins

Applicable Instruments

MTQ064F:It is compatible with various nucleic acid extraction instruments such as Singu32, EXM 3000 nucleic acid extractor by Zybio.

MTQ096F:It is compatible with various nucleic acid extraction instruments such as Singu96, KingFisher Flex System by Thermo Fisher, EXM 6000 by Zybio.

Applicable Test Items

Suitable for test items for simple samples, such as novel coronavirus, respiratory pathogens, etc.

Specimen Requirements

Pharynx swab, nasal swab, nasopharyngeal secretion, serum, plasma, cervical exfoliated cells.

Applications

RT-PCR, cDNA synthesis, Northern/ dot/ slot blot analysis, Microarrays, RNAseq

Product Performance Indicators

Extraction sensitivity:The amplification sensitivity reached ≥100 copies/mL.

Nucleic acid purity:A260/A280 ratio: 1.5~2.2.

Extraction recovery: nucleic acid extraction recovery rate reached 90%~110%.

Whole Blood Nucleic Acid Extraction Reagent (Magnetic Bead Method)

Ref No. &Specifications

l MTQB064 (64 Tests/Kit)

l MTQB096 (96 Tests/Kit)

Features

l Optimal performance for extracting DNA from complex samples

l Easy to handle: Proteinase K solution can be stored at room temperature for more than 12 months, eliminating the need for manual preparation.

l Easy and fast operation, ideal DNA can be obtained in 24 minutes.

l High efficiency and precision: High concentration and purity of nucleic acid products, without inhibitors, 1.6 < A260/A280 ≤ 2.1, 2-10ug of genomic DNA in 200μL of whole blood, can fully meet the downstream detection applications, especially for high sensitivity nucleic acid quantification, such as HBV and HCV ultra-sensitive nucleic acid detection.

l Wide applicability: It can extract viral DNA/RNA from a variety of samples such as swab wash, plasma/serum, whole blood, virus culture, tissue fluid, urine, secretion, vaccine, environmental gauze, etc.

l High compatibility: Applicable to the existing NP968-C, NP968-S, GeneRotex 96 and PANA9600S nucleic acid extraction platforms of Tianlong.

l Good batch-to-batch stability, with intra-batch CV <10% and inter-batch CV <15% of extracted gene purity, and high experimental reproducibility.

Main Components

Storage Conditions and Validity

The kit is transported and stored at 15-30℃ and valid for 12 months.

The kit contains a Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for 1 year stored at 2~30℃.

Extraction Time

24-30 mins

Applicable Instruments

MTQB064: It is compatible with various nucleic acid extraction instruments such as Singu32 or Singu48.

MTQB096:It is compatible with various nucleic acid extraction instruments such as Singu96, KingFisher Flex System by Thermo Fisher.

Applicable Test Items

Suitable for qualitative DNA test items.

Specimen Requirements

Fresh or frozen whole blood.

Applications

Nucleic acid test of human genome and human mutant gene, bacterial and viral DNA test in blood

Product Performance Indicators

Average yield (200μL of blood): ≥2μg of Genomic DNA.

Nucleic acid purity:OD260/OD280 ratio: 1.6~2.1.

Extraction recovery:nucleic acid extraction recovery ≥90%.

Precision:the in-batch concentration coefficient of variation is ≤10%.

Bacterial Nucleic Acid Extraction Reagent (Magnetic Bead Method)

Ref No. &Specifications

l MTQM064 (64 Tests/Kit)

l MTQM096 (96 Tests/Kit)

Features

l Optimal performance for extracting DNA from complex samples

l Fast extraction: Minimum of 21 minutes to complete the whole process of DNA/RNA extraction.

l Biosafety: No lysis at high temperatures, reducing the formation of aerosols thus reducing the risk of infection to professionals. Does not contain toxic organic reagents such as phenol and trichloromethane.

l Reduced cross-contamination: The reduction of aerosols significantly reduces cross-contamination and false positive results.

l High sensitivity: a positive sample is diluted many times and still high quality viral nucleic acid can be extracted.

l Good reproducibility: good reproducibility of the extraction of the same sample

Main Components

Storage Conditions and Validity

The kit can be transported and stored at 15~30 ℃ with a validity period of 12 months.

Extraction Time

21 mins

Applicable Instruments

MTQM064: It is compatible with various nucleic acid extraction instruments such as Singu32 or Singu48.

MTQM096:It is compatible with various nucleic acid extraction instruments such as Singu96, KingFisher Flex System by Thermo Fisher.

Applicable Test Items

Suitable for qualitative DNA test items.

Specimen Requirements

Sputum, samples from male urethra/female urethra/female cervix/female vagina, cervical exfoliated cells, stool specimens, rectal swabs and anal swabs, bronchoalveolar lavage fluid, throat swabs, oropharyngeal/oral secretions, nasal/nasopharyngeal secretions

Applications

RT-PCR, cDNA synthesis, Northern/ dot/ slot blot analysis, Microarrays

Product Performance Indicators

Average yield: Extract 200μL specimens, nucleic acid ≥1μg.

Nucleic acid purity: OD260/OD280 ratio: 1.7~2.1.

Extraction recovery: nucleic acid extraction recovery is ≥85%.

Precision: the coefficient of variation of in-batch concentration is ≤10%

Veterinary Nucleic Acid Extraction Reagent (Magnetic Bead Method)

Ref No. & Specifications

l MTQ064A (64 Tests/Kit)

Features

l Optimal performance guarantees for DNA extraction from complex samples

l Super paramagnetic beads and buffer system with unique separation action

l The powerful protein denaturant contained in the lysate rapidly dissolves the protein and dissociates the nucleic acid.

l Wide applicability: Viral DNA/RNA can be extracted from various types of animal samples, such as whole blood, serum, plasma, saliva, nasal fluid, urine, feces, tissue, feed, and environmental samples.

l Good inter-batch stability, intra-batch precision CV≤5%, high experimental reproducibility.

Main Components

Storage Conditions and Validity

The kit can be transported and stored at 10~30 ℃ with a validity period of 12 months.

Extraction Time

21 mins

Applicable Instruments

Singu32 Nucleic Acid Extractor from Singuway Biotech Inc.

Applicable Test Items

Suitable for veterinary test items.

Specimen Requirements

Whole blood, serum, plasma, saliva, nasal fluid, urine, oral and nasal swabs, environmental gauze, fecal swabs, animal tissues, feed pellets.

Applications

Aquatic and livestock samples for extraction, enrichment and purification steps of nucleic acids. The processed products are used for clinical in vitro assays.

Product Performance Indicators

Extraction recovery:90%~110% recovery of nucleic acid extraction.

DNA integrity:the extracted DNA bands were single, clear, with appropriate fragment size and no trailing bands or obvious degradation.

Intra-batch precision:ct value, intra-batch CV value ≤ 5%.