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Singuway Nucleic Acid Detection Kit
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Home Products Veterinary PCR Detection Kit
Ruminant Disease

This product is used for the rapid detection and screening of animal diseases. The series of products have the advantages of simple and rapid, high sensitivity and high specificity, and can be used for instant detection of disease-causing microorganisms in livestock, aquatic animals, pets, feed, water and even the environment.


Package specification: 48 tests/box


Storage conditions and shelf life.

Store at -20±5°C away from light, valid for 12 months. Shipped on dry ice. Repeat freeze-thaw less than 5 times. Date of manufacture, expiry date see outer packaging box.


Product Features:

Short response time, short waiting time.

Easy to operate and quick to interpret.

It is highly sensitive and less likely to cause false negatives.

High specificity, the designed primers are specific enough to amplify the detection target without causing amplification of other pathogenic microorganisms, reducing the appearance of false positives.


This product is for research use only and the test results cannot be used as the sole basis for clinical diagnostic judgment.

Test

Internal Control

Abbreviations

Pathogen Category

Target Gene

Sample Type

Required Channels

Minimum Sensitivity (Copies/Ul)

Brucella

Exogenous

Brucella

DNA pathogens

National Standard

Vaginal discharge, effusion products, fetal clothes, semen, penile discharge, eye, nose and mouth swabs, breast milk, urine, whole blood, etc.

FAM; VIC

2.5

Peste Des Petits Ruminants Virus

Exogenous

PPR

RNA virus

N gene

Nasal swabs, oral swabs, eye swabs, and tissues such as lymph nodes, spleen, kidney, rectum, colon, and lung, etc.

FAM; VIC

2.5

Bovine Viral Diarrhea Virus Type 1

Exogenous

BVDV1

RNA virus

5UTR

Blood serum, tissue samples, nasal specimens, semen, milk, etc.

FAM; VIC

2.5

Bovine Viral Diarrhea Virus Type 2

Exogenous

BVDV2

RNA virus

5UTR

Blood serum, tissue samples, nasal specimens, semen, milk, etc.

FAM; VIC

2.5

Sheep Poxvirus, Goat Poxvirus and Bovine Nodular Dermatosis Virus

Exogenous

SPPV, GTPV

DNA virus

serine protease inhibitor-like SPI-2

Take biopsies or autopsy of cattle and sheep skin papules, lung lesions, lymph nodes, blood, etc.

FAM; VIC

1

Sheep Poxvirus, Goat Poxvirus and Bovine Nodular Dermatosis Virus Multiplex

Exogenous

LSDV

DNA virus

serine protease inhibitor-like SPI-2

Skin, lymph nodes, diseased lung tissue, spleen, diseased nodules on organs and peri-lesional tissue, etc.

FAM; VIC

1

African Horse Sickness Virus

Exogenous

AHSV

RNA virus

VP7 gene

Whole blood, tissue, etc.

FAM; VIC

2

Bcsp31 Gene and Virb12 Gene of Brucella  Multiplex

Exogenous

Brucella+A19 virB12

DNA pathogens

bcsp31+virB12

Vaginal discharge, effusion products, fetal clothes, semen, penile discharge, eye, nose and mouth swabs, breast milk, urine, whole blood, etc.

FAM; VIC; ROX

2.5

Bovine Viral Diarrhea Virus

Exogenous

BVDV-U

RNA virus

5UTR

Blood serum, tissue samples, nasal specimens, semen, milk, etc.

FAM; VIC

2.5

Akabane Virus

Exogenous

AKAV

RNA virus

Conservative gene

Blood, viscera or muscle samples, etc.

FAM; VIC

5

Mycobacterium Bovis Paratuberculosis

Exogenous

MAP

DNA pathogens

NP gene

Feces, milk, intestinal segments and lymph nodes, etc.

FAM; VIC

1

Bluetongue Virus

Exogenous

BTV

RNA virus

NS3 protein gene

Heparin anticoagulated blood, organs (spleen, liver, kidney, lymph nodes), semen, etc. of animals

FAM; VIC

5

Mycoplasma Bovis

Exogenous

MPB

DNA pathogens

ATP-binding cassette domain-containing protein

Tissue samples, nasal swabs, milk samples, joint fluid samples, etc.

FAM; VIC

2.5

Bovine Contagious Pleuropneumonia (Mycoplasma Mycoides)

Exogenous

MmmSC

DNA pathogens

lipoprotein

Joint fluid, pleural effusion, nasal swabs and whole blood, joint fluid and thoracic exudate, etc.

FAM; VIC

5

Eastern Equine Encephalitis Virus

Exogenous

EEEV

RNA virus

NS1 protein

Blood, cerebrospinal fluid, tissue, etc.

FAM; VIC

2.5

équine Infectious Anemia Virus

Exogenous

EIAV

RNA virus

gag protein

Blood

FAM; VIC

5

Equine Piroplasmosis

Exogenous

Epi

DNA pathogens

K54 gene

Blood, tissue, etc.

FAM; VIC

5

Infectious Bovine Rhinotracheitis Virus

Exogenous

BoHV-1/IBR

DNA virus

gB gene

Serum, whole blood, swabs, semen, tissue samples, etc.

FAM; VIC

5

Border Disease Virus

Exogenous

BDV

RNA virus

5UTR

Serum, tissue samples, aborted fetuses, fetal membranes, amniotic fluid, and secretions and excretions from persistently infected animals

FAM; VIC

5

Getah Virus Multiplex

Exogenous

GETV

RNA virus

Specific conservative gene

Tonsils, lymph nodes and other tissue lesions

FAM; VIC

5

Bovine Viral Diarrhea Virus and Classical Swine Fever Virus Multiplex

NoInternal Control

BVDV/BDV/CSFV

RNA virus

Specific conservative gene

Serum, tissue samples, nasal specimens, semen, aborted fetuses, fetal membranes, amniotic fluid, and secretions and excretions from persistently infected animals

FAM; VIC; ROX

5

Bovine Epidemic Fever Virus

Exogenous

BEFV

RNA virus

Specific conservative gene

Whole Blood

FAM; VIC

5

Isospora and Eimeria(Multiplex  Fluorescence PCR)

Swine Endogenous

Iso/Eim

DNA pathogens

Specific conservative gene

Feces

FAM; VIC; ROX

5

Porcine Reproductive and Respiratory Syndrome Virus Type Classical North America(Fluorescence RT-PCR)

Exogenous

PRRSV-U

RNA virus

NSP2 gene

Lungs, spleen, kidneys, tonsils, lymph, saliva, blood and semen, etc.

FAM; VIC

5

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